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This observation cautions for direct comparison of mutant lines even when they are bred in the same facility.
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In contrast, in the mutant line even one generation of inbreeding already caused a significant decrease in fecundity (p = 0.023), and by generation four fecundity had been reduced by 46.8percentt in the mutant.
It is remarkable that these general alterations are observed even in mutant lines that are deficient in steps early or late in the desaturation pathways.
Surprisingly, even the AtrabD2b and AtrabD2c single mutant lines produce aberrant pollen at a level of about 10%.
This effect was even more pronounced in the triple mutant lines, where crater-like structures developed with occasional cracks in the epidermal cell layer (Fig. 2c).
(c) Sanger sequencing of mutant lines.
We initially tested whether rsl4 mutant lines disrupt the low P root hair elongation response.
These isogenic WT and mutant lines were differentiated to D30_L using our established strategy (Fig. 1a).
However, in the quadruple deletion mutant lines, cell protrusion was delayed (Fig. 5c).
The mutant lines used in this study are: fls2 (SAIL_691C4) desi3a-1 (SALK_151016C).
ΔPpdrm2 and ΔPpdnmt3a single deletion mutant protoplasts were used to generate ΔPpdrm1ΔPpdrm2 and ΔPpdnmt3aΔPpdnmt3b double deletion mutant lines, respectively, as described above.
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