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Table S2 summarizes mapping data for mutant lines discussed in this paper.
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As with the previous two studies discussed above, the genetic analysis of ACS mutant lines suggests a model in which ACC or some derivative of ACC may act as a signalling molecule.
Though the authors did not explicitly discuss the findings, correlation of an increased MPK4 activation in ap2c3 mutant lines and MPK4 expression in stomata suggests that MPK4/AP2C3/AP2C1 module regulates stomata aperture.
Jeng et al. (2012) discovered mutant lines that have higher Fe or Zn concentrations in polished seeds by searching among NaN3-induced mutant lines (Oryza sativa cv. IR64).
Such differences between wild type and mutant have been discovered efficiently in recent years by using NGS sequencing of bulked DNA of mutant F2 progeny from crosses between mutant lines and parental lines in several plant and animal systems.
Requesting the mutant lines involves a complicated process such as MTA and quarantine.
The homozygous overexpressor and knockout mutant lines were confirmed using western blot analysis.
Instead, most groups focus on the characterization of specific mutant lines.
Consistent with this observation, Hd3a mRNA accumulation is lower in se5 hd1 than in se5 mutant lines.
(B) Phenotypes of RNAi mutant lines expressing OsPDS RNAi HpRNA construct.
a Relative expression of R2R3-MYBs genes in WT, OsSND2-OX and snd2-c1 mutant lines.
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