Finally, we have been able to visualize the telomere RNAs at different ovarian stages of development in wild type and mutant lines, demonstrating their presence in spite of being tightly regulated by the piRNA mechanism.
Molecular characterization of this mutant line demonstrated that rps19 deficiency reproduced the erythroid defects of DBA, including a lack of mature red blood cells and p53 activation.
In vitro experiments on m.1555A>G mutant cell lines demonstrated that exposure to aminoglycoside would impair growth; however, not all symptomatic individuals have been exposed to aminoglycoside.
In preclinical studies, a subset of resistant BRAFV600E mutant cell lines demonstrated increased CRAF signalling via BRAF/CRAF heterodimerisation and resulted in a shift from B-RAF to CRAF dependency.
Both mutant tumor cell lines demonstrated metabolic sensitivity to the drug, confirming their feasibility for [18 F]FDG-PET imaging of RO5126766 efficacy.
In line with the lack of effect of VHL expression on p27 levels in A498 (as seen in Figure 2B), VHL expression had no effect on the morphology of A498 cells, with VHL-negative, wild-type VHL, and all VHL mutant cell lines demonstrating a disorganized fibroblastic phenotype.
Previous and current screens of mutant lines have demonstrated that perturbation of ion channel physiology and cardiogenesis also impacts the incidence of heart failure [7] [9; Dowse et al, 1995; K.O., L. Qian and R.B. unpublished].
A detailed quantitative comparison of the rod phenotypes between the two cell lines demonstrated that mutant huntingtin cell lines were significantly delayed in rod formation during stress, and that rods were typically fewer and longer, with a persistent phenotype (Supplementary Material, Table S1A).
Physiological analysis of atocd and atocd/p5cs1-4 mutasts as well as AtOCD RNA lines demonstrated that lack of AtOCD expression significantly increased in proline accumulation at low ψw in a manner that was independent of P5CS1.
