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Such embryonic lethals are predicted to make up at least 30% of all mutant lines created, based on preliminary data and bibliometric analysis from The Jackson Laboratory (JAX) Mouse Genome Informatics (MGI) database (Table 1).
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The double mutant lines were created by cross-pollination between the relevant mutants, and putative double mutant plants were screened from the phenotype segregation ratio of the F2 progeny.
Here we describe a mutant zebrafish line created by TALEN genome editing carrying a 23 bp deletion in gba1 (gba1 c.1276_1298del), the zebrafish orthologue of human GBA1.
Cell lines created from IDH mutant gliomas have been reported to eliminate their IDH mutation under standard culture conditions [ 7].
Were the lines created for irrigation?
Notice how the lines created differ.
Given the multiple variability factors, assay comparisons between full-length Cx43-expressing REKs and each individual mutant REK cell line created were performed using a t test with a statistical cut-off at P<0.05.
add_vline lines Creates a vertical line.
add_hline lines Creates a horizontal line.
Hatching: Single, straight lines create shadows.
Four hundred and forty-five independent mutant lines of flies were created; approximately 8% of these (35 of 445) were homozygous lethal.
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