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In conclusion, a TILLING population of 2550 mutant lines containing a high frequency of mutations is presented.
Consequently, we examined exocyst mutant lines containing labeled auxin transport proteins, PIN1-GFP, PIN2-GFP, PIN7-GFP, AUX1-YFP, and ABCG36-GFP to see if mislocalizations were evident.
A double recessive homozygous mutant derived from ss1 and ss3a null mutants was sterile; however, double recessive mutant lines containing a leaky ss1 mutant and a ss3a null mutant were fertile, as were mutant lines in which one or both of these genes were heterozygous with the wild-type allele.
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By contrast, double pul and isa1 mutant lines contained higher levels of WSPs and had shorter amylopectin chains with DP ≤7 in the endosperm compared with the sug-1 parents, indicating that PUL can partly compensate for starch biosynthesis.
Wildtype and mutant lines contain a similar range of large sense transcripts.
Peripheral cells of the kinesin-13a-1 mutant lines contained a few large vacuoles, but few vesicles (Fig. 3A).
It was also indicative that, despite SOD-deficiency, both mutant lines contained superoxides and H2O2 levels in their photosynthetic part/s quite effectively under As treatment.
Two other mutant lines contained base substitutions in the following splice sites: T to A mutation in a donor splice site of intron 21 (M1032 line) and A to G mutation in that of intron 10 (M1033 line; Fig. 2b).
The durum wheat mutant line containing the splice junction mutation had a lower resistant starch level of 4.7 or 6.2% in the flour consistent with the lower amylose levels in this mutant line.
One mutant line, containing a T-DNA insertion in the sixth exon of the gene, was identified in the superpools and then in the pools, and kindly provided by INRA.
The o2 mutant line contained o2m(r), a null expression O2 allele, while the o7 mutant was obtained from the Maize Genetics Stock Centre at the University of Illinois (Urbana-Champaign) [ 54].
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