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Figure 1 A-C shows SXRF analysis of Cu (A) and Fe (B) localization in the wild type and indicated mutant lines compared to total fluorescence density (C).
Consistent with this observation, red1-2 seeds accumulate less protein, and the expression of two late embryo development reporter transgenes, LEA::GUS and β-conglycinin::GUS, was significantly weak and started relatively late in the red1-2 mutant lines compared to the wild type.
The intensity of exflagellation (as a measure of male gametogenesis) was not altered in the pf16 mutant lines compared to wild type parasite (Figure 1A), however these parasites displayed a severe defect in male gamete motility and fertility, with 50% of male gametes being immotile (Figure 1B,C).
Nuclear-encoded Lhca1 was increased in all HL-grown mutant lines compared to the wild type.
The changes in cell wall composition observed in the mutant lines compared to the wild type indicate that LRX proteins indeed have a function in cell wall formation.
Seed weights from field grown plants were reduced in both the wild-type sibling and the high amylose mutant lines compared to the non-mutagenized parent lines (Table 5).
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Kohel et al. observed restricted and similar fiber elongation pattern for the mutant lines, comparing Li 1 and Li 2 with TM-1 in a fiber developmental study [ 11].
There were no changes in the level of HAM2 mRNA in ham1 mutants lines compared with control.
Interestingly, IRE1α was abundantly expressed in mutant p53 cell lines compared with wild-type p53 cell lines.
Using densitometry, the average levels of Toca-1 relative to β-actin was significantly higher in mutant p53 cell lines compared to WT p53 cell lines.
By co-IP we observed an increased interaction between the NFs in mutant HSPB1 cell lines compared to non-transduced or HSPB1-WT controls (Fig. 2a d).
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