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The Tos17 mutant collection consisting of approximately 50,000 mutant lines characterized into flanking sequences have been used in full characterization of many genes characterized in Japan and nearly 50 Tos17 mutant lines have been successfully used in tagging specific genes (See reference list at https://tos.nias.affrc.go.jp/doc/references.html).html
We identified at least 25 mutant lines characterized by a variety of reproducible phenotypes, including cardiac and nuchal edema, neural tube defects, a truncated anterior-posterior axis, situs inversus or the absence of limbs and lungs (Table 1; Figure 2).
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The mutant lines were characterized with respect to potyvirus resistance and translation of mRNA with the objectives to get insights into molecular mechanisms underlying translation initiation factors-mediated resistance to potyviruses.
Liu et al. [ 20] found that the Arabidopsis xpd mutant lines were characterized by growth defects, decreased UV resistance, and limited efficiency in excision of UV photoproducts.
The phenotypes of the two mutant lines were characterized by a reduction of kernel size in the gln1.4 mutant and by a reduction of kernel number in the gln1.3 mutant.
In contrast to the null defects identified previously [ 4], RT-PCR analysis showed that each of mutant lines we characterized produced cals5 mRNA, although levels were reduced in cals5-5, pereflectingectinefficientient splicing of intron 5, due to the large T-DNA insertion (not shown).
YKa designed this study, produced the AtYLMG1-1 overexpressing and knockdown lines, produced the SYlmG1 overexpressing and gene disrupted lines, characterized the phenotypes of mutant lines, and drafted manuscript.
AM produced the AtWhy2 and AtWhy2-myc overexpressing lines, characterized the phenotypes of the mitochondrial Whirly mutants, designed the study and drafted the manuscript.
One double homozygous mutant line was characterized by RT-PCR to confirm that the normal mRNA for both of the genes was absent (data not shown), and this particular Atss2, Atss3 line was used in all further analyses.
† Indicates lines characterized in greater detail.
In this study, 20 novel bmr mutant lines were identified and characterized based on allelism tests.
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