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Whereas, genetically non-balanced microscopes of mutant lines after tetrad dissipation were unable to further development.
Finally, we studied FUS localization in WT and mutant lines after differentiation to ventral spinal cord neural cells for 34 days (Fig. 2), in control conditions and during stress.
The relative amounts of amylose and amylopectin in starch granules were compared between wild type and the three mutant lines after separating starch polymers by gel permeation chromatography (GPC).
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Indeed, no phosphorylation was detected in a GCN2 null mutant line after branched and aromatic amino acid deprivation.
For the esk1-4 mutant line, after 10 days of treatment the PLA reduction reached 76% compared to plants grown in standard conditions and for esk1-5 mutant lines there was a reduction of 62%.
Two different apoptotic responses and patterns in control and mutant lines were found after nocodazole treatments.
All the mutant lines, which were kept after the two rounds of screen, were used in complementation crosses.
It was found also that different nocodazole concentrations rapidly induced apoptotic processes in both control and mutant lines, but cell responses after nocodazole treatments were completely different.
TLA was calculated for the homozygous mutant lines and wild type after 7 days of averaged mild water deficit and control conditions (Table 2).
We named this mutant line Mariusz (after the world's strongest man at the time, Mariusz Pudzianowski).
Interestingly, these mutant lines showed less accumulation of H2O2 after PAH exposure.
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