PVY-LYE90 and PepMoV-Texas RNAs were never detected in inoculated or non-inoculated leaves of the G1485A mutant line, indicating that viral accumulation is impaired at an early stage of the infection process (Figure 2B).
The lack of full-length CRWN transcripts from homozygous mutant lines indicates that all four mutations used in this study are likely to be loss-of-function alleles.
The absence of Wolbachia in the other two snz mutant lines indicated that this endosymbiont was likely not responsible for the snz mutant lifespan extension.
Analysis of the bread wheat homozygous mutant lines indicated that they had a higher amylose content of 55% compared to wild-type sibling lines of 23% amylose (Table 5).
Mass spectrometry and Western blots did not corroborate transcriptional differences in the mitochondrial proteome of the MSC mutant lines, indicating that post-transcriptional events, such as protein longevity, may compensate for reduced transcription in MSC mitochondria.
In our model it can be observed that AtPrx55 expression level is affected in all these mutant lines, indicating a good candidate for lignification of siliques except that the gene was down-regulated by SHP1 and SHP2.
Whereas the 3D7pHBIMFwt line responded to increases in blasticidin concentration by substantially upregulating msp1-f transcript levels, likely via increases in episome copy number (confirmed by copy number estimation, data not shown), much less upregulation was seen in the mutant lines, indicating an inability to respond to elevated drug concentrations.
In-gel kinase activities of two independent CPK quadruple mutant lines indicate that the Ca2+-activated kinase signals are CPK-derived (compare lanes 9 10 with 13 16 in B and see Figure 3 figure supplement 1 ); predicted MWs for CPK1, CPK2, CPK5, CPK6, CPK11, and CPK23 are 68.3 kDa, 72.3 kDa, 62.1 kDa, 61.1 kDa, 55.9 kDa, 58.7 kDa, respectively.
Black line indicates location of GGG to ATA mutations in mutant transposon ITR.
Red line indicates model fit.
