Your English writing platform
Discover LudwigExact(6)
Genetic diversity creation is a core technology in directed evolution where a high quality mutant library is crucial to its success.
Another novel addition to this insertion mutant library is the inclusion of unique barcodes.
The lower-end estimate for the quadruple mutant library is that ∼20 000 compounds might be accessible.
Genomic DNA from the TMDH mutant library is digested using an appropriate restriction enzyme and amplified using linker PCR.
The initial construction of the mutant library is labour-intensive but, if well-constructed, it should only be necessary to perform this step once.
Similarly, although the quadruple mutant library is obviously undersampled (although it is still the largest of its kind reported to date), one could estimate that between 16 000 and 48 000 compounds could be produced using this methodology.
Similar(54)
Using a cloned copy of the IS 6100 mobile element [ 36], a transposon mutant library was constructed.
Sensitivity analysis of gene knockout mutant library was further performed (phenomics data).
Thus, high-throughput screening of PHB synthase mutant library was available using LipidGreen1 through 96-well microtiter plate-based assay.
The methods that are involved in creating a mutant library are extensive and can be divided into several categories according to their basic ideas.
A novel and effective mixture culture method was proposed and used to realize the whole mutant library being high-throughput screened after mutagenesis.
More suggestions(4)
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com