Sentence examples for mutant library consisting from inspiring English sources

Tn insertion mutants used for genotypic and phenotypic analysis of biofilm formation, were obtained from a sequence-defined transposon mutant library consisting of 1,952 strains, each containing a single mutation within a nonessential gene in strain USA300 JE2 [ 8] obtained from the NARSA repository (www.beiresources.org/).org/

Typical mutant libraries consisted of more than 108 independent clones.

From a library consisting of 2126 knock-out mutants, we identified 3 mutants with high-flux and 95 mutants with low-flux phenotypes that did not have severe growth defects.

Fig. 5 Fluorescence intensities of the mutant library, which consisted of a pool of phaC genes with PCR-introduced random mutations.

To this end, we infected HMEC-DD-NeuT cells (lacking the mutant PIK3CA) with subpools of a kinome-wide retroviral library consisting of 354 human kinases and kinase-related open reading frames (Boehm et al., 2007).

The Keio collection, a non-essential gene library consisting of 3985 single-gene deletion mutants of E. coli K-12 Bwas113 [42], was grown at 37°C overnight in 100 µl of LB medium in 96-well plates without shaking.

A reduced representation ApeKI library consisting of 21 Ethylmethane Sulfonate (EMS) induced mutants and two wild type cv.

This led to a virtual library consisting of 2500 cells.

A library consisting of two individual siRNA sequences targeting human kinases were obtained from Qiagen (v.1.0).

We generated a HGMEC SAGE library consisting of 68,987 transcript tags.

In contrast, the loop library consisted of mainly mutant sequences through five rounds of infection, and these mutant viruses continued to thrive despite the presence of wild type clones in the fifth round library.