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A comprehensive transposon mutant library comprising 10,080 independent clones was constructed by electrotransformation of the restriction-deficient derivative of strain ATCC 13032, C. glutamicum RES167, with an IS 6100-containing non-replicative plasmid.
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The two mutant libraries comprising about 9000 transformants were screened with the Winkler test.
This method generated a TMDH library comprising around 106 mutants.
We screened a deletion library, comprising ∼84% of all non-essential fission yeast genes, for drug-resistant mutants.
Next, we obtained a focused siRNA library comprising several known clock components.
Only one 3DS-specific BAC library (TaaCsp3DShA, [ 20]) is available at present and this library, comprising 36,864 clones, was used.
A single colony filter per library comprising 55,296 clones was probed (additional file 1).
Clones exhibiting one to three substitutions in the TAS2R16 coding region were selected to create a comprehensive mutation library comprised of 573 mutant TAS2R16 plasmids with substitutions in all 291 residues.
The Library comprises of 25 libraries, including the Doe/Moffitt Libraries, The Bancroft Library, the C. V. Starr East Asian Library, and numerous subject specialty libraries.
The SG-1000's game library comprises 68 standard cartridge releases and 29 Sega Card releases.
The resulting library comprised ca 23,000 BAC clones.
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