Sentence examples for mutant libraries containing from inspiring English sources

Stocks of the different transposon mutant libraries containing 20% glycerol were prepared from the overnight cultures.

After mating and selection, the resulting haploid double mutant libraries containing individual gene deletions with either mst2∆ or epe1∆ were mated again, and haploid triple mutants were selected on YES medium supplemented with antibiotics to measure cell growth.

Therefore, it is currently difficult to create a mutant library containing amino acid substitutions in more than seven residues.

Compared to the wild-type enzyme, two mutants Gly267Ser and His134Arg/Phe141Leu with 1.14- and 3.30-fold increased catalytic efficiency (kcat/Km) toward locust bean gum (LBG) were obtained from the mutant library containing 19,700 clones.

To overcome this problem, here we have used two different TNF mutant libraries, each containing random substitutions at six selected amino acid residues, and utilized a gene shuffling method to construct a randomized mutant library containing substitutions at 12 different amino acid residues of TNF.

The STM technique was adapted to barcode individual transposon mutants and a large mutant library containing >29 000 independent clones was created using a set of 27 signature tags.

The power of the redesigned phage-based transposition system was demonstrated through the preparation of a transposon mutant library containing ~694,000 independent insertions in the S. aureus HG003 strain background (Additional file 2).

In each target composition, [Term] = 0. "Natural": the library with the natural abundance (the average composition in 207 natural proteins) [18]; "No Cys": the library with uniform composition except [Cys] = 0; "c-Fos e', g'-": a c-Fos mutant library containing only 4 kinds of amino acid as same as in Ref.[26].[26]

Our mutant library contains approximately 10 mutational variants, assessed by counting colony-forming units after efficient E. coli transformation.

We present evidence for piggyBac element remobilization in the latter parasites and therefore such parasites can be effective tools for generation of mutant parasite libraries containing random mutations.

Currently, directed approaches for evolving a Uaa-specific RS involve the generation of an initial RS mutant library minimally containing 10 to 10 members.