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To facilitate direct comparison between the two methods, the samples were analyzed at 1% mutant level when using CADMA.
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In this case, the chance of resistance emerging from the mutant merozoite seeing residual drug level when released from the liver is much greater, although the numbers of parasites exposed to residual levels and their individual survival probabilities are much lower.
This double mutant, however, induced similar IL-18 level when compared with H37Rv Δ esxA.
The observed correspondence between Dichaete and Sox2 target genes show that these proteins are not only conserved at the functional level when assayed in mutant rescue experiments but also, remarkably, at the level of the gene regulatory networks they control in the fly and mouse nervous system.
When mutant levels are increased, the stalling is increased and results in reduced mtDNA levels.
The osu1 mutants had higher anthocyanin levels when C increased to 15 or 30 mM.
Production was restored to wild-type levels when each mutant strain was complemented with the respective gene, demonstrating that the phenotype of the Δ sven0914 mutant is not simply the result of a polar effect of the deletion of sven0915 and that both genes play a role in CHL production.
Oddly, we could not detect any discernible change in Cyclin E levels when cic mutant clones were generated in an rbf1 120a homozygous background despite the presence of ectopic S-phase cells.
Two of the studied mutants, G109C/Basal mutant and R135C/Basal mutant, regenerated with 11-cis-retinal to different levels when compared to the WT (Table 1).
Interestingly, the R103W mutant showed significant loss of mitochondria-encoded COX2 levels when compared with wild-type and P486S mutant (Fig. 4F).
Rather, the elevated CTP levels and the observation that growth became particularly affected at mid-log phase support the hypothesis that the CtpSE277R mutant is defective in regulating CTP levels when adapting to changes in the cellular environment.
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