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We next explored potential mechanisms underlying the delayed onset of metamorphosis in feeding black mutant larvae given a rapamycin diet relative to the starved larvae.
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dC1GalT1 mutant larvae have also been shown to have morphological defects in the CNS.
During development lef1 mutant larvae generate intercalary neuromasts, but not as many as control larvae.
In order to determine whether there was a similar mechanism behind the delay in metamorphosis in fed black mutant Manduca, wing imaginal discs were removed from black mutant larvae, which were subsequently given rapamycin- or DMSO-treated diets.
Moreover, the number of somatic cells expressing P granules was dramatically increased in lin-35; lsy-2 mutant larvae (Fig. 4C E).
Indeed, regarding the cuticle, we observe a phenotypic series from strong defects in gho mutant larvae, over moderate defects in hau mutant larvae to weak defects seen in sar1 mutant larvae.
As in hau and gho mutant larvae and consistent with recently published data [19], the ER of the epidermal cells of sar1 mutant larvae is spherical.
A few muscles were absent in the mutant larvae (Figs. 3F and G), and many muscles were absent in rare cases of mutant larvae (Fig. 3H).
Wild-type or mutant dauers and growing L2/L3 daf-c mutant larvae were always assayed in parallel with growing L2/L3 wild-type larvae at the test temperature.
This could account for the hyperthermic rescue of Mys localization in Rac1 mutant larvae.
Free oligosaccharides (fOSs) were isolated from the soluble fractions of wild type- or mutant larvae.
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