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Hypomorphic homozygous dark CD4 mutant larvae derived from its homozygous stock had an average of two vCrz-sib neurons (Fig. 10F).
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To test whether Drosophila mutant for DmWRNexo are similarly sensitive to CPT, larvae derived from crosses of CG7670 e04496 heterozygotes were propagated on medium supplemented with varying concentrations of CPT, or vehicle-only control (0 µ m CPT).
Majority of the larvae derived from irradiated eggs had an abnormal appearance.
To improve section plane consistency in sets of larvae, we have developed new array casting molds based on the outside contours of larvae derived from 3D microCT images.
The authors go on to say that " However, since PolIIo ser2 levels can occasionally be reduced on chromosomes derived from chd1 mutant larvae, CHD1 activity may indirectly impact transcriptional elongation".
dC1GalT1 mutant larvae have also been shown to have morphological defects in the CNS.
Moreover, the number of somatic cells expressing P granules was dramatically increased in lin-35; lsy-2 mutant larvae (Fig. 4C E).
Indeed, regarding the cuticle, we observe a phenotypic series from strong defects in gho mutant larvae, over moderate defects in hau mutant larvae to weak defects seen in sar1 mutant larvae.
As in hau and gho mutant larvae and consistent with recently published data [19], the ER of the epidermal cells of sar1 mutant larvae is spherical.
A few muscles were absent in the mutant larvae (Figs. 3F and G), and many muscles were absent in rare cases of mutant larvae (Fig. 3H).
Wild-type or mutant dauers and growing L2/L3 daf-c mutant larvae were always assayed in parallel with growing L2/L3 wild-type larvae at the test temperature.
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