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The linked rgh*-00S-005-14 seed mutant lacks a 6PGDH isozyme activity suggesting pgd3-umu1 causes an enzymatic knockout.
The mutant lacks a highly conserved Walker A box lysine residue, which binds the β- and γ-phosphates of ATP.
The Arabidopsis thaliana catalase2 (cat2) mutant lacks a peroxisomal catalase and under photorespiratory conditions accumulates H2O2, which leads to activation of cell death.
As the hAR mutant lacks a large amino acid sequence, we synthesized a small peptide derived from the AR 622-670 acido acid sequence responsible for interaction of the receptor with FlnA.
Recall that mutant MA1 contains S149D and S153D mutations to partially reintroduce the Mn2+A site; however, this mutant lacks a histidine metal ligand that would ordinarily complete the Mn2+A coordination polyhedron in arginase.
The pcrA mutant lacks a functional perchlorate reductase and thus was unable to grow anaerobically with (per chlorate but was still able to grow aerobically and anaerobically with nitrate (Bender et al. 2005).
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A comparison between the pH rate profile for wild-type FlK and pH dependence studies of the FlK-H76A mutant led to the conclusion that the mutant lacks an ionization at acidic pH.
Conversely, the truncation mutant lacking a portion of the fusion domain was unable to promote these steps.
Typhimurium A9 (mutant lacking a 15kB fragment spanning recA-hydN genes) formed more abundant biofilms, which were not responsive to treatment with nitric oxide donor molsidomine.
Interestingly, these mAbs changed their binding also to other mutant, lacking a transmembrane anchor-Gln1230Stop.
Similar experiments could be done for transverse vs. axial motion comparing wild type to model organisms such as the myosin-XVa mutant lacking a staircase architecture.
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