Due to unspecific contacts, some CadC clusters were even detected in a mutant lacking the CadC-binding site20.
A K. pneumonia mutant lacking the pathogenic factor was used as the host strain.
In addition, in a mutant lacking the CadC-binding site, most cells (81%) did not form a CadC cluster, even under CadC-activating conditions (Fig. 6b).
Consistent with this, an UNC-45 mutant lacking the UCS loop (UNC-45ΔUCSloop) is less efficiently cleaved by the protease (Supplementary Fig. 5a).
However, they were detected as faint diffuse density distal to the Dl tier in a difference image between wild-type ClpAP and a mutant lacking the N-domain.
To this end, we generated a novel cell line DT40-SWΔC, which conditionally expresses a C-terminus-truncated AID mutant lacking the nuclear export signal.
Here, we show that an Arabidopsis mutant lacking the receptor-like kinase FERONIA FERR) shows severely altered Ca2+ signaling and growth responses to different forms of mechanical perturbation.
A double mutant lacking the PMI and PMM genes produced 8-deoxyamphoteronolides in good yields along with trace levels of glycosylated amphotericins.
A JAM-A mutant lacking the first Ig-like domain necessary for homophilic binding localized to cell cell contacts similar to wild-type JAM-A.
Our genetic analysis of a double mutant lacking the function of both complexes in the same pathway showed that high-B sensitivity was not additive in the double mutant compared with either single mutant (Supplementary Fig. 15).
Studies on a VEGF-C mutant lacking the C-terminal propeptide (vegfc um18) suggest a role of the C-terminal propeptide in lymphatic development5, and a similar mutation was found in a patient with Milroy-like lymphedema6.
