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Conversely, the truncation mutant lacking a portion of the fusion domain was unable to promote these steps.
Typhimurium A9 (mutant lacking a 15kB fragment spanning recA-hydN genes) formed more abundant biofilms, which were not responsive to treatment with nitric oxide donor molsidomine.
Kane, WH, Devore-Carter, D, and Ortel, TL. "Expression and characterization of recombinant human factor V and a mutant lacking a major portion of the connecting region". Biochemistry 29, no.
Interestingly, these mAbs changed their binding also to other mutant, lacking a transmembrane anchor-Gln1230Stop.
Similar experiments could be done for transverse vs. axial motion comparing wild type to model organisms such as the myosin-XVa mutant lacking a staircase architecture.
Furthermore analysis of a mutant lacking a functional TTSS3 showed that when bacteria are unable to escape from the phagosome they become strongly co-localised with LC3, confirming that LC3 is recruited to B. pseudomallei-containing phagosomes.
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In our study we used the strain CC503, a nit1 nit2 mutant lacking an active nitrate signaling pathway (nit2).
For controls, culture supernatant from the wild-type strain and an inactive T6SS mutant lacking an essential component, Δ tssE, was also probed.
Moreover, the ycf1Δ mutant lacking an ABC-type transporter responsible for sequestration of metal-glutathione conjugates into vacuoles [ 27- 29] was also As and Cd sensitive.
The mutant lacks a highly conserved Walker A box lysine residue, which binds the β- and γ-phosphates of ATP.
The linked rgh*-00S-005-14 seed mutant lacks a 6PGDH isozyme activity suggesting pgd3-umu1 causes an enzymatic knockout.
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