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It was possible that this mutant lacked the ability to fold the protein properly.
However, conidia of this mutant lacked the typical yellow-brown pigmentation.
The zrt1zrt2ZHY3 mutant lacked the Zrt1 and Zrt2 proteins for Zn uptake [ 41, 42].
This BRCA1 mutant lacked the BRCT repeat region believed to be involved in DNA repair [ 4, 5].
The fet3fet4DEY1453 mutant lacked the Fet3 and Fet4 proteins and was defective in both low- and high-affinity Fe uptake [ 40].
Southern blot analyses showed that the sltB2 mutant lacked the entire predicted AN6132 coding sequence in a deletion of at least 9 kb (data not shown).
Similar(53)
Due to unspecific contacts, some CadC clusters were even detected in a mutant lacking the CadC-binding site20.
A K. pneumonia mutant lacking the pathogenic factor was used as the host strain.
In addition, in a mutant lacking the CadC-binding site, most cells (81%) did not form a CadC cluster, even under CadC-activating conditions (Fig. 6b).
Consistent with this, an UNC-45 mutant lacking the UCS loop (UNC-45ΔUCSloop) is less efficiently cleaved by the protease (Supplementary Fig. 5a).
Our genetic analysis of a double mutant lacking the function of both complexes in the same pathway showed that high-B sensitivity was not additive in the double mutant compared with either single mutant (Supplementary Fig. 15).
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