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While deletion of SET2 had little effect on the internal TSSCs identified in the single upf1∆ mutant, it revealed new Set2-sensitive 'A' and 'B' TSSCs.
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A careful analysis of the mutant strain revealed that it had acquired the ability to utilize glutamate as the only source of carbon and energy.
Cloning of one such mutant revealed it to be an allele of the β-spectrin encoding gene sma-1.
Indeed, studies using a hog1 Δ mutant strain revealed that, although it depends on the severity of the stress, ∼80% of the genes that are induced upon osmostress depend on the Hog1 MAPK for full induction (Posas et al. 2000; Rep et al. 2000; O'Rourke and Herskowitz 2004; Capaldi et al. 2008).
However, among all the mutants tested, the areA mutant revealed the most severe growth defects.
Detailed characterization of the Asp-280-Ala mutant revealed that it retains a functional reductase domain, as measured by its ability to reduce cytochrome c.
Analysis of Chp from the CG10166 mutant revealed that it exhibited no obvious physical phenotypes.
Further investigation of the D469G mutant revealed that it only functions in multiple copies when cells are exposed to forms of DNA damage that result in base modifications or strand breaks.
Characterization of the CTD-NΔ35 mutant revealed that it has a Kcat/Km value of 0.0362 µM−1 min−1, which is approx. 72% of the wtCoaE that contains both the NTD and the full length CTD.
Leaf-movement assays on the flm mutant revealed that it had no circadian defect (P = 0.69).
Biochemical characterization of the mammalian Gαi2[G203T] mutant revealed that it can still bind Gβγ and GTP, but upon nucleotide exchange Gαi2[G203T] fails to adopt the activated confirmation and can further lose GTP (Inoue et al., 1995).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com