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The thermodynamic stability of the I430A mutant is reduced relative to wt-KaiC; CD melting experiments indicate that the Tm of the mutant is lowered by about 3°C relative to wt-KaiC.
The activity of the Phe300Gln mutant is reduced >200-fold.
Blebbing in the end3 -1 ras2 Δ double mutant is reduced to 3.5 blebs/colony.
Oleic acid inhibition of the R53E mutant is reduced threefold, while R61E is reduced fivefold.
Indeed, the binding of this mutant is reduced compared to WT MutL.
Furthermore, the germination rate of the ascospores from the Δsec22 mutant is reduced.
Similar(41)
In contrast, the Km value of CoA for K172A mutant was reduced by ∼3.5 times (Fig. 6D).
When expressed from a CEN plasmid to ensure physiological levels, binding of the S98A mutant was reduced strongly at ADH2 compared to wildtype Adr1 (Figure 4).
In contrast, the Prn-ko mutant caused similar disease severity relative to the wild type, however, colonization of the Prn mutant was reduced relative to the wild type during early and late infection.
Hapfelmeier et al reported that the residual inflammation caused by infection with a SPI-1 mutant was reduced significantly if mice had a mutation in MyD88, which abrogates most signaling through TLRs [27].
Compared to EGFR-WT, Cbl association to the 1045+ mutant was reduced by four- to five-fold, while association with the 1068/86+ mutant was reduced to negligible levels.
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