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Collectively, our results suggest that the increased skin disease burden exhibited in ODDD patients harbouring the fs260 mutant is probably due to multiple additive effects cause by the mutant during epidermal differentiation.
As such, the reduced level of H3 K56Ac in the H4G94P mutant is probably related to the reduced total amount of H3/H4 rather than a defect in the acetylation reaction per se.
This indicates that attenuation of the FAM53 mutant is probably not due to an alteration of the cell envelope, so it might be that a metabolic defect is responsible for the reduced initiation of granuloma formation.
Considering that a glutamine can provide the same hydrogen bonds, the 15-fold decrease in kcat,app for the E408Q mutant is probably due to perturbation of the electrostatic environment of the active site.
However, as indicated by the short-termed appearance in blood on day five this mutant is probably still able to colonize the primary niche (i.e. the vascular endothelium) (Dehio, 2004; Schröder and Dehio, 2005).
Another reason for the involvement of QA in PSII in higher superoxide production in PsbS mutant is probably the shift of redox-potential of QA to a more negative value due to the lack of PsbS, similar to the A249S mutant of Thermosynechococcus elongatus [ 83], which can make QA− to be able to reduce the molecular oxygen.
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Their high levels in the ST mutant were probably associated with higher sugar levels detected in these plants.
The shortened seed phenotype of the srs3 mutant was probably the result of a reduction in cell length in the longitudinal direction [ 25].
Thus the changes in voltage-dependent activation in this mutant are probably due to alterations in channel gating rather than a direct effect on the putative voltage-transduction mechanism.
On the other hand, the lower shoot Pi and Po observed in the longer term in the shoots of this mutant are probably a direct consequence of the decreased P concentrations observed in the roots.
Hence the PRC observed in our double mutants is probably caused by changes in the circadian pacemaker.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com