However, industrial strains are rarely auxotrophic, and screening for spontaneous or induced auxotrophic mutants is necessary (cf. infra).
Moreover, current results give only an incomplete understanding of their association, and further study of the lifespan and stress response of various IIS pathway mutants is necessary.
More in vivo studies comparing the tumorigenic events that occur with specific VHL mutants are necessary to fully understand their differential risks of RCC.
To identify yeast mutants with a point mutation, detection of the specific mutant alleles is necessary.
Therefore, monitoring for resistance relies on measuring infection half-life (White 2011), and to accurately detect a new resistance mutant, it is necessary to quantify how much variation is present prior to drug-imposed selection.
Huntington's disease is deemed a genetic condition on both the individual and population accounts: a single mutant gene is necessary, and arguably sufficient given necessary (and standard) background conditions, for symptoms to appear in a given person; the presence and absence of disease symptoms in members of the population is accounted for in terms of the presence and absence of the mutation.
Furthermore, using a cardiac-specific inducible TG system [13], we found that continuous expression of the mutant protein is necessary for sustaining the high concentrations of the amyloid oligomer that correlate with depressed cardiac function as well as premature lethality [14].
Clonal expansion of mutant cells is necessary for the accumulation of additional mutations and subsequent tumor formation.
This phenotype is linked to a mobile substance as the bps1 mutant root is necessary to induce arrest of bps1 shoots, and in graft chimeras, the bps1 root is sufficient to induce arrest of the wild-type shoot [ 9].
In the Men system, only a single mutant allele is necessary because complementation (up-regulation of Men ) is tested in Men −/ Men + heterozygotes, allowing us to cross Men − alleles with essentially any third chromosome with a functional copy of the Men gene.
