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In addition, we had also shown that an etk knock-out mutant is much less resistant to polymyxin than a wild-type E. coli K-12 strain.
The calculated energy state of the mutant is much lower, causing a self aggregation.
However, the phenotype of the cyp85A2 mutant is much less severe than that of the brassinosteroid insensitive bri1 mutant [ 19].
The double mutant is much smaller and yellower than either of the single mutants at 22°C.
As for DNA methylation profiling with MET module, the CG methylation in met1 mutant is much lower compared to WT.
This can be interpreted as an expression of the fact that the cellular metabolism in the Δ rel mutant is much more unbalanced.
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The catalytic efficiency for each active mutant was much higher than that of wild-type at low pH.
The optimal concentrations of glucose and yeast extract for cordycepin production of the mutant was much higher than that of control (wild strain) and the cordycepin production was 2.79 times higher.
The induction in the mutant was much slower and less substantial.
Nuclear localization of the G39A mutant was much reduced in general and less than 20% showed nuclear speckles (Figure 6A-C).
Moreover, the mutant was much more diffuse throughout the cytoplasm of cells with few to no puncta, and exhibited a prominent reticular pattern suggestive of ER localization.
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