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R178X mutant is missing the fold back structure (α5, α6) and flexible C-terminal of XLF (Fig. 8D).
When fully bound, GltPh-R397A crystallizes in the outward-facing state, like wild type GltPh, except that L-asp coordination is slightly altered because the mutant is missing the key coordinating side chain of Arg397.
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Each mutant was missing a gene thought to be involved in biofilm formation.
The result showed that the mutants were missing many neurons, consistent with previous findings (data not shown).
To summarize the main text, Rtls mutants are missing both the bright staining at the center of rosettes (because the mutants do not produce rosettes) and the fainter, cell-membrane associated patches found in wild type single cells and chains.
In the first mutant, AP3 is missing.
A more elaborate discussion of the simulation results is presented in Additional file 2. In the second mutant, PI is missing.
To investigate which effectors were involved, we examined the Δ core mutant that is missing tir, map, espF and eae (Intimin) genes and is also defective for the expression or secretion of additional effectors including EspH, EspZ, NleH, NleF and NleA (Ruchaud-Sparagano et al., 2007).
D36 mice express a mutant protein that is missing the PKA binding domain but continues to bind and localize other signaling molecules including the phosphatase, calcineurin.
In the current study, we have dissected the early events that follow ligand binding to the CXCR4 receptor to identify deficiencies stemming from a mutant CXCR4, which is missing the 19 carboxy-terminal residues and is associated with WHIM syndrome.
In the hrs1 deletion mutant, the rMTOC is missing and γ-tubulin accumulation and rMT formation are abrogated.
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