The emp2-R mutant was linked to an albino mutant (w3) such that the loss of Emp2 could be seen as white somatic sectors.
PCR and Western blots confirmed that the transformant was fully segregated and all HoxF in the mutant was linked to GFP (ESI Fig. 2 and 3 †).
Thus, whereas EPEC-mediated TER decreases are linked with TJ disassembly (Simonovic et al., 2000; Dean and Kenny, 2004; Guttman and Finlay, 2009), the tir mutant was linked with extensive cell detachment with up to ∼80% of the monolayer destroyed, compared with just ∼10% for WT EPEC.
Thus, if the previously described attenuated virulence phenotype in similar mutants is linked to CupD-dependent attachment or biofilm formation, it suggests that cupD gene expression and signaling through the rcs/pvr two-component systems only occur in vivo, or in particular environmental conditions, which were not mimicked by our in vitro biofilm assay.
These findings suggest that the hyper- or hypo-secretion phenotype of these mutants is linked to cellulase secretion (Additional file 4: Figure S7A,B).
To evaluate whether the observed non-conventional activation of STAT factors by the oncogenic mutants is linked to their mislocalisation, we investigated the signalling capacities of PDGFRα-D842V in the presence of Brefeldin-A (BFA).
Analysis of the results allows us to hypothesize that the increased hydrolytic ability of the mutants is linked to (i) improved ligand binding in a putative secondary binding site, (ii) the diminution of surface hydrophobicity, and/or (iii) the modification of thumb flexibility, induced by mutations at position 111.
Notably, suppression of APC/C mutants was linked to loss of Ubp8 catalytic activity and required histone H2B ubiquitination.
We found that higher histone acetylation in rpd3 mutants was linked to local changes in chromatin structure, leading to a more open conformation at the affected replication origins.
