In this study, the wild type was sensitive to both photoperiod and temperature; however, the mutant was insensitive to photoperiod but sensitive to temperature.
The H4G94A mutant was insensitive to these agents, whereas the H4G94P mutant was sensitive to HU, but not to MMS or Zeocin.
EGFR/D770insNPG, the most active mutant, was insensitive to gefitinib (0.1 μ M, 5 h), as were the less active mutants EGFR/D770InsNPH and EGFR/M766InsASV.
This effect was mediated by residue C361, since a Brf2 C361A mutant was insensitive to treatments with the alkylating agent and displayed unaltered affinity for TBP/DNA complexes.
The inhibitory effect of naringenin was conferred by the expression of the TRPP2 protein, because the pkd2 − mutant was insensitive to naringenin-dependent reduction in cell behaviour.
As shown in Figure 4B, root elongation in the ΔAtFAAH mutant was insensitive to both 0.1 and 100 μM OdDHL (8) exposures, exhibiting no significant increase or decrease in total length at these concentrations, respectively.
The formation of the ternary complex was highly sensitive to Brf2 treatments with GSSG/GSH, an effect that was fully reversed by addition of reducing agents and again exclusively mediated by C361, since the C361A mutant was insensitive to treatments with high GSSG/GSH ratios.
These mutants are insensitive to brassinolide yet still respond to tomato systemin by producing protease inhibitors and causing an alkalisation response.
fca and fve mutants are insensitive to ambient temperature and flower at the same time under different temperature conditions (23°C and 16°C).
