Sentence examples for mutant is indeed from inspiring English sources
Equilibrium and kinetic experiments demonstrate that folding of the ΔP174 mutant is indeed three-state.
If cell cycle progression in the FMP mutant is indeed altered, then we hypothesize that FMP43 protein is related to and somehow affects key regulatory mechanisms governing the cell cycle, as for instance the G1/S-phase control point.
How did the authors determine that this mutant is indeed nucleotide-free when purified?
As shown in Figure 2B, immunoblotting confirmed that the T51A mutant is indeed expressed and to the same extent as the WT PAGE4.
These results indicate that the UV-induced sterility of the rad2C65Δ mutant is indeed the result of defective actin dynamics due to the deletion of 65 amino acids in the Rad2p C-terminal region.
The phospho-mimetic S62D-Bcl-xL mutant is indeed less efficient than the corresponding phospho-deficient S62A-Bcl-xL mutant in sequestrating Bax and in protecting cancer cells from mitotic cell death or yeast cells from Bax-induced growth inhibition.
Thus, to further validate that wildtype and the SPI-1 mutant are indeed localized inside 3-D HT-29 cells, we performed a differential immunofluorescence microscopy staining technique that allows one to distinguish between extra- and intracellular bacteria [45], [46].
Western blotting of cytoplasmic and nuclear fractions confirmed that the FAK-NLS mutant was indeed defective in nuclear localization.
To validate the SEC observations and to confirm that the oligomerization state of the triple mutant was indeed concentration-dependent, we turned to a combination of analytical ultracentrifugation and SEC-MALS.
The V419S sensor mutant was indeed able to interact with the ORAI1 R91W mutant, and this interaction was accompanied by a substantial decrease of FRET close to the plasma membrane, consistent with full-length STIM1 observations (Muik et al, 2008; Derler et al, 2009).
Furthermore, to test that the slow development and low embryonic survival of the nrf-5 mutant was indeed due to the nrf-5 sa513 nrf-5 sa513 we took adVantage of the mutationtrain RB786 containing a large deletion in the gene stdh-1 located 0.09 c m awey from nrf-5 on chromosome V.
