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Information on the generation and complementation of a Δafoch4 mutant is given in Supplementary Figure S4.

Before mutant genes are cloned, each mutant is given its own name, but renaming may be necessary once the causative mutation is identified.

However, if the there is an interaction between the two, then the observed effect of the double mutant is given by 3where Δ GI represents the coupling free energy.

Process data of double knock out mutant is given in Additional file 3. Three cultivations were performed; the only difference between the first and the two last was the fructose isotopomer composition used in the C-labelled feed.

Mutagenic primers used to obtain each mutant are given in Table 1.

This mutant was given the name MinnGold because of its bright yellow leaf coloration during early foliar development.

Eleven close contacts, exposed before emergence of the mutant, were given oseltamivir prophylaxis on the patient's fourth day of treatment, and they remained well.

The average structural branchpoint distances of 1000 suboptimal structures sampled from within 5% of the MFE for each mutant are given in Table 2.

The average GFP fluorescence lifetime of transfected cells with and without cotransfection of Cdc42 mutants are given in table (Fig. 3 (v)).

The model parameter setting for the wildtype simulations as well as for the simulations of mutants are given in Table 1 and Table 2.

The distributions of computed structural branchpoint distances for each of the RPS17B mutants are given in Figure 1.