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A complementation test performed by crossing pgr7 with npq4, a mutant lacking PsbS [29], showed that the PSBS gene in the pgr7 mutant is functional (Figure S2).
Importantly, this mutant is functional: ectopically expressed T286A assembled with CDK4 in cultured cells and showed similar levels of kinase activities to wild type cyclin D1 (data not shown; [ref. 7]).
We are also in the process of defining the shortest deletion mutant that could confer resistance to DT and ETA: preliminary results indicate that the same human Dph2 deletion mutant is functional but not a shorter version with a 133 amino acid deletion (data not shown).
Other reports support an active monomeric form of SecA, indicating that the monomeric SecA mutant is functional, whereas a disulfide cross-linked dimer is nonfunctional and its reduced monomeric counterpart functional.
Can the authors show that the interaction is important for PCVII to exit the ER? (i.e., identify a mutant in either TANGO1 or SLY1 that disrupts the interaction, and test whether this mutant is functional for PCVII ER export).
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The R667A mutant was functional and exhibited virus entry activity similar to that induced by wild-type SARS-CoVpp, albeit at a slightly lower level (Figure 4D).
Each time an assayed mutant was functional, we sequenced the new P450 gene.
Nonetheless, the single sperm cell-like cell in fbl17 mutants is functional but will exclusively fertilize the egg cell of the female gametophyte, giving rise to an embryo that will later abort, most likely due to the lack of functional endosperm.
All mutants were functional and did not differ from one another with respect to current amplitude, steady-state to peak ratio, or magnesium block.
However, when we also over-expressed Ubc9 in these cells this was able to drive the modification of Pc2 by both of the SUMO mutants, to a level similar to that seen with the wild type proteins, suggesting that these SUMO mutants are functional for substrate modification (Figure 7A).
First, all 19 KCNQ2 mutants are functional, whereas only 15 Kv1.2-2.1 mutants are functional.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com