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No tolerance is observed when the transport null Athma4(D401A) mutant is expressed (Figure 1A).

This vesicle size is reminiscent of that observed when the Rab5-GTP mutant is expressed in HeLa cells [19].

Our findings not only support the conclusions of the previous study done with a different mouse model, but also directly show that constant light quite efficiently rescues photoreceptor degeneration when the Ca2+ -insensitive GCAP1 mutant is expressed at the levels similar to that of the wild type GCAP1, whereas constant dark exacerbates the disease.

We have previously shown that the C24A mutant is expressed normally on the plasma membrane of transfected cells (20).

Approximately 75% as much of the N-glycosylation mutant is expressed as the WT AC8 (Fig. 4E)].

This mutant is expressed in the nucleus and retained ∼10%15%% residual double-strand ligation activity, but was barely detectable in the patient [O'Driscoll et al., 2001].

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The deglycosylated triple mutant was expressed and had binding properties similar to those of glycosylated FGFR4ed, but was still heterogeneous.

The wild-type Grx2 and the C15S mutant of Grx2 (the C15S mutant) were expressed with the C-terminal Histag (LEHHHHHH).

Two independent clones for each mutant were expressed and analyzed.

The cytosolic 518-Ynd1 mutant was expressed at relatively low levels, but could still be efficiently coimmunoprecipitated with E4orf4.

Each mutant was expressed in E. coli and purified to near homogeneity via a C-terminal His-tag.

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