Expression of XHSF380, a dominant-negative HSF1 mutant, was embryonic lethal, which could be circumvented by delaying expression via a tetracycline inducible promoter.
TEAD1 null mutation is embryonic lethal and the mutant mice suffer from severe heart defects.
In contrast, Pik3c3 homozygous mutants are embryonic lethal and die between E7.5 and E8.5 of embryogenesis.
In mice, analysis of the relative contribution of Mkk4 and Mkk7 to JNK activation has been complicated by the fact that Mkk4 and Mkk7 single mutants are embryonic lethal [2].
cpl-1 (a cathepsin L cysteine protease) mutants are embryonic lethal and defective in yolk processing, but lethality may be due to yolk platelet aggregation in the cytoplasm rather than an energetic requirement for yolk [25].
As homozygous fkh mutants are embryonic lethal [15], we applied the GAL4/UAS system to either knock down FKH in larval tissues by RNA interference or to raise FKH levels by ectopic overexpression, each under different nutrient conditions.
Since pros null mutants are embryonic lethal, we first studied mitotic activity in prosv24, a strong hypomorphic allele [27] that reduces PROS expression in the larval OLs (Fig. 3A, B).
Indeed, while mouse ACC1−/− mutants are embryonic lethal [6], [7], mutant ACC2−/− mice have a normal lifespan but higher fat oxidation rate in muscle and adipose tissues and are resistant to diet-induced obesity [8] [10], observations which have made ACC2 an attractive target for treatment of obesity and type 2 diabetes [7], [11] [13].
Homozygous mutants are embryonic lethal between E7.5 and E9.5.
Ago1 nonsense mutants were embryonic lethal, although they showed normal axis patterning (data not shown).
