The polymorphisms, C (wild-type) and T (mutant), are displayed in the box on the template sequence (3' to 5' direction).
Single mutants were displayed on the surface of EBY100 cells using Frozen-EZ YeasTransformationon II Kit and plated on selective SD-CAA solid agar media.
This library of mutants was displayed on the surface of phage and challenged with protease Arg-c to select stably folded proteins.
EETI-II mutants were displayed on the surface of yeast as fusions to the agglutinin mating proteins under the control of a galactose promoter, and contained a C-terminal cMyc epitope tag for detection and quantification of knottin expression levels using an anti-cMyc antibody.
All 11 mutants are displayed schematically in Figure 1.
Clustered annotation data for G2 mutants are displayed in Figure S3.
The 10 most enriched Fcab mutants were displayed on yeast again and individually analyzed for binding to Her2-ECD (3 nM) at pH 6.0 and 7.4.
In Figure 2b, the luciferase activity of each mutant construct is displayed as a percentage of the activity of the wild-type CbxU283 construct.
For each gene, a summary of the mutant phenotype information is displayed on the Locus Summary page, and the complete information is displayed in tabular format on the Phenotype Details Page.
