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The mutant is devoid of pro-MMP-2-activating capability but is highly effective in blocking MT1-MMP-mediated FITC-labelled collagen and gelatin film degradation in HTC75 fibrosarcoma and MCF7 breast cancer models.
The Y to F mutant is devoid of enzyme activity whereas the Y to H mutant possesses ∼5% wild-type oxidase activity; their properties are compared with those of wild-type enzyme.
The Zmtsb1 Zmtsb2 (orp1 orp2) double mutant is devoid of β-activity and shows severe growth defects [ 15].
The lpcat1 mutant is devoid of any significant oil phenotype compared to its null segregant or to WT. Crosses of AS11 (dgat1) with the lpcat1 yielded progeny homozygous for both mutations.
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This mutant was devoid of any C-domain activity.
Likewise, the H169A/D171A mutant was devoid of the ability to perform 2OG turnover.
Likewise, when Lys103 was replaced with Ala (the residue that is conserved at this position in OspF), the resulting VenL-ΔC mutant was devoid of measurable lyase activity.
We also observed that some abnormal pollen grains from the AtrabD2b/2c double mutant were devoid of nuclei, as indicated by DAPI staining, whereas all pollen from wild-type and single mutant plants (data not shown) have nuclei.
Finally, the nodules of the V1 and exoY mutants were devoid of any bacteria (data not shown) in agreement with the results of histochemical staining.
These results did not correlate with the transactivation activity of HIV-1 Tat since wild type HIV-1 Tat, and the RGD domain mutant exhibited transactivation activity while the remaining mutants were devoid of this activity (not shown).
The pGL3-77/105A and pGL3-92/105A mutares are devoid of all base pairs with probability >0.5 between nucleotides +77 to +105, indicative of abolition of structure in this region of the RNA.
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