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It is assumed that the sucrose uptake rate observed into the suxA-deleted mutant is determined by slow and passive diffusion of sucrose across the outer membrane, which is sufficient to support growth in the presence of 20 mM sucrose in the medium.
As illustrated in Figure 4A, the EOT ensemble of each mutant is determined cotranslationally as TMDs exit the translocon.
Statistical significance between wild type and each mutant is determined by two-tailed Student's t -test (*p<0.05; *p<0.051; *p<0.05001).
Our genetic analysis conclusively show that the glossy phenotype of the GL mutant is determined by a single Mendelian locus, BnaA.GL.
The fixation probability of a newly arisen mutant is determined by its fitness change (selection coefficients) and effective population size, which are concepts adapted from population genetics [ 68- 70].
The proposed mechanism predicts that the final topological distribution of each EmrE mutant is determined by both the distribution of configurations in the EOT ensemble and the available post-translational kinetic pathways that lead to fully integrated protein topologies.
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Here, the structure of this P47H mutant was determined in complex with NADH to 2.5 Å resolution.
The sequence of the disrupted ORF for each attenuated mutant was determined by either cloning or PCR-amplifying and sequencing the flanking regions.
Crystal structures of the mutant were determined in two space groups, P21212 and C2, from crystals grown in the same crystallisation set-up.
The flgE mutant was determined to harbor a single transposon insertion after hybridization with the transposon vector pUTminiTn5Km2 (Table 1) (data not shown).
In vitro growth rate of S. epidermidis 1457 and its isogenic ACME-excision mutant was determined in tryptic soy broth as measured by OD600.
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