The phenotype of the infected bos1 mutant is complex.
However, we note that the majority of the genes with siRNA changes (either increase or decrease in hrde- 1 mutant) have similar mRNA expressions between the hrde- 1 mutant and WT in each of the four phases (Fig. 5c, d, e; Additional file 7), suggesting that the connection between the siRNA and mRNA expression changes (WT v.s. hrde- 1 mutant) is complex and may be limited.
Again, the effect of TLC1(Ku)3 in these mutants was complex, with some telomere shortening evident.
The relationship between the value of epistasis and the relative mutant frequency is complex and determined by the fitness ranking of individual mutants.
However, gene identification via forward mutant screen is complex, as FN-induced mutagenesis hits multiple targets.
When we compare the phenotypes reported for the SadA, SibA, talin, paxillin, Rap and RapGAPs with the one of secG−, we find that the phenotype of the secG mutant is less complex and we cannot easily link SecG to any of theses proteins.
The strain lineage of the D15 mutant is rather complex, therefore, we used a bulk segregant approach, which narrows down the genomic region responsible for the phenotype.
We have tried to improve the discussion of this mutant, which, we realize, is complex, as it entails the interaction with PP2A, the possibility that the deletion removes an important regulatory intramolecular interaction, etc.
While the changes in cell identities in mutant lobes are complex, the discovery of heterogeneity in the medullary zone populations of anterior and first posterior lobes is consistent with recent reports that this population has distinct fate-restricted cell populations (Krzemien et al., 2010; Minakhina and Steward, 2010).
Clearly sensorimotor gating and social interaction, those behavioural domains most sensitive to PNS in the Bdr mutant, are highly complex tasks that require the integration of many brain circuits (97, 98), although studies are only just beginning to isolate which neurodevelopmental pathways are most susceptible to environmental factors (52, 99).
Dendritic tree in cul3 mutants is more complex with significantly increased branch density.
