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Previous studies have described similarities between the lipid profiles (especially the components of PAT, DAT and sulpholipids) of the phoP mutants and H37Ra [40], [41], [41] – although it is unclear if the attenuation of the phoP mutant is comparable to that of H37Ra.
Similar to the results seen in the cell lines (Figures 4E and 5A), the levels of phospho-STAT5 in bone marrow infected with the triple mutant is comparable to that seen in wild type relative to the vector only control (Figure 6A,B).
We show that the generation time of this mutant is comparable to the wt.
Moreover, the rmsd of the mutant is comparable to wild-type NdCld.
Under O2 saturating conditions, the calculated kcat of the R90K mutant is comparable to that of wt, demonstrating that the mutation of Arg to Lys provided full HEPD activity at higher 2-HEP concentrations.
In contrast, the level of activity seen with the GA mutant is comparable to that of the GA/US mutant in this line and both constructs are significantly less active than in the MCF-7 line.
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Budding kinetics of the mutant were comparable to those of the wild type (Figure 3A).
In general, neutrophil accumulation by the ΔLF/EF mutant was comparable to the positive 3% thioglycolate control (Fig. 6D).
Remarkably, the CRX interaction of the adRP-linked p.G56R mutant was comparable to that of the NR2E3 wild-type protein.
The rescue ability of the D-CblL-mPR mutant was comparable to that of D-CblS (90∼100%).
The number of acid-fast negative cells developed in the complemented strain (C-Δ-tgs1) of Δ-tgs1 mutant was comparable to the wild type (Fig. 11A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com