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The D172A pvADA mutant is bound even more tightly (Ki = 0.9 μM).
The dramatic cotyledonary phenotype of the double mutant is bound to have secondary consequences on seedling growth, making it very difficult to dissect primary from secondary effects.
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Furthermore, only 3% of C-set peaks of the NKX2-5YRDY−A mutant were bound by ELK1/4.
Likewise, only ∼3% of the genes with expression changes in the TF mutant were bound by the TF.
This is in good accordance with the in vitro data which showed that this mutant was bound to CHO and HEK cells and efficiently secreted into the cell culture medium.
Comparison of the percentage of FITC-positive macrophages showed that WT M. bovis BCG and the SapM and capping mutants were bound or taken up by murine macrophages with similar efficiency (Suppl. Fig 3A).
Arg was immobilized on Affigel 10 beads (Bio-Rad) according to the manufacturer's directions, and GST-tagged Arg fragments and mutants were bound to glutathione agarose beads at a concentration of 20 μM (moles of protein per volume of beads).
Performing pull-down assays with human brain extracts, they showed that the phosphorylated form of PKR is bound to mutant HTT mRNA.
We compared NKX2-5 WT and mutant peaks and adopted a simple nomenclature (A, B, and C) for peak subsets, where A is bound by NKX2-5 WT only, B is bound by both WT and a mutant, and C is bound only by a mutant.
As Figure 5C shows, wild type and K767R mutant ADNP were bound to H3K9me3 peptide in a HP1β dependent manner.
Because sera obtained from KID mice exhibited IGF1 bioactivity similar to that of control sera (by KIRA assay), we believe that the concentration of Des-IGF1 that we measured using the above RIA mostly represents the fraction of Des-IGF1 mutant that was bound to the IGFBPs (with lower affinity than wild type).
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