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In keeping with this, the wadC mutant is attenuated in dendritic cells and mice.
Consistent with these findings, ΔrhlR and ΔrhlI mutants have radically different biofilm phenotypes and the ΔrhlI mutant displays full virulence in animals whereas the ΔrhlR mutant is attenuated.
This implies that the hfq mutant is attenuated in both the macrophage and epithelial cell lines (Figure 1B).
An rfaC mutant is attenuated in an animal model potentially through affecting complement binding and serum-mediated killing [61].
Expression of the plc-genes is upregulated after infection of macrophages and the quadruple knock out mutant is attenuated in growth in lungs and spleen of mice.
Indeed, an M. tuberculosis cysH mutant is attenuated and generates protective efficacy against tuberculosis infection equivalent to that of BCG [55].
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The auxotrophic NK41 mutant was attenuated as determined by the increase of the LD50 after intraperitoneal challenge.
Also the tpx mutant was attenuated in a mouse peritonitis model [26].
Also in Helicobacter pylori, a crucial role of Ggt has been identified since a mutant was attenuated in both piglets and mice [66].
These observations demonstrated that the virulence of the kerV mutant was attenuated in the mouse lung infection model and thus aroused much less inflammation.
Our results showed that the relA spoT mutant was attenuated for virulence and induced protective immunity by s.c. vaccination against bubonic and pneumonic plague.
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