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Indeed, the sigV single mutant is as sensitive as the oatA/dltA double mutant, and the sigV/oatA/dltA triple mutant displays the highest level of lysozyme sensitivity suggesting synergistic effects of these genes.
This kind of genetic interaction, in which the double mutant is as viable as any of the single mutants, usually indicates that the pair of proteins belongs to the same complex and/or operates in the same pathway [31].
Supplementary Figure S2F shows that dpb11 WG700,701AA point mutant is as defective as dpb11Δ C in checkpoint activation and the inability to activate Mec1, therefore, correlates which a deficiency to support checkpoint signalling.
We have mutated Phe89 that has been shown to stack with adenine in ADP-complex (Bhatt et al., 2010), to Ala and show that the mutant is as active as the wild-type PfDTD.
Curiously, sreA null mutant is as virulent as an A. fumigatus wild-type strain, but HapX-deficiency causes significant attenuation of virulence in a murine model of aspergillosis [ 86].
Surprisingly, the K41A mutant is as competent as wild-type Srs2 in inhibiting D-loop extension, indicating that the helicase and/or translocase activities of Srs2 are not required for this inhibition.
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The ΔpvdD mutant was as fit as the wild type in pure culture, and the ΔpvdDΔpchEF mutant slightly and significantly fitter, but both mutants were outcompeted by the wild type in mixed culture.
Surprisingly, also the mprF insertional mutant was as resistant as the JH2-2 parental strain (Fig. 2A).
As apparent, D159A mutant showed 20% decrease in activity whereas K189A mutant was as active as the wild type enzyme.
As shown in Fig. 1C, the I464A and R466A mutants, as well as the double mutant, are as functional as wild type Chk1.
The ΔrelA Y. pestis mutant was as virulent as wild type Y. pestis KIM5+ (Fig. 6), while the ΔrelA ΔspoT Y. pestis mutant was dramatically attenuated.
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