Sentence examples for mutant into the from inspiring English sources

As an immediate practical application we report the expression of a complete functional monoclonal antibody expressed from a single polypeptide, which was cleaved by our TEVp mutant into the two antibody chains and secreted as an assembled and functional molecule.

We then inserted a GFP Tyr151TAG) mutant into the same plasmid under the Mtb hsp60 promoter to afford plasmid pSMT-MjtRNA-GFP151TAG (Figure 1A).

We then asked the question whether the interaction between SPT7L and TAF8 would be maintained and thus SPT7L would take the TAF8 mutant into the nucleus (Fig. 3A).

Toward this end, we insertionally inactivated BA3443, BA0672, and BA1295 in a ΔSterne/Wip4 background and inoculated each mutant into the worm microcosm.

After the initial screening, candidate mutants were tested again for AHL responsiveness in triplicate using a more precise procedure in which molten motility agar is seeded with a liquid overnight culture of the mutant in question, rather than stabbing the mutant into the wells (see Materials and Methods).

Since both the PilC1 mutant and the wild-type strain induced similar proinflammatory responses (Figure 6B), it is still possible that entry of the PilC1 mutant into the CSF may be a result from sepsis-induced cell damage of the BBB.

To determine the consequences of disrupting AtRAD21 (which has been associated with HR) in a C-NHEJ DNA repair pathway mutant, we've introgressed the atrad21.1 mutant allele into the atku80 mutant background [ 46] to produce the atrad21.1 atku80 double mutant.

To ask whether this elevated Wg expression contributes to the defects in the mutant, we introduced wg mutant alleles into the miR-965 (KO1/KO2) mutant background.

To determine the extent to which each of these genes alters FLP-7 secretion properties, we crossed the respective null mutants into the FLP-7mCherry secretion line.

Internalization of S. aureus eap and sae mutants into the MAC-T epithelial cells was significantly decreased compared with the wild-type counterparts.

However, surprisingly, we found that forcing the K122 acyl mutants into the mitochondria with an IMS-targeting tag did not recover their ability to suppress respiration.