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Second, the model was determined from the D96G/F171C/F219L triple mutant instead of a photointermediate of wild-type bacteriorhodopsin.
Involvement of the latter mechanism is supported by the markedly reduced levels of APP mRNA found in infected cells 18 h p.i. and by the increased transcript levels observed when cells were infected with the vhs-deficient HSV-1 mutant instead of wild-type HSV-1.
The production and selection of a revertant virus is achieved exactly in the same way, but in this case the cells are infected with the deletion mutant, instead of the wild-type virus, and transfected with a plasmid containing a full-length wild-type copy of the gene and its flanking regions.
A similar result was obtained using the cim3-1 mutant instead of MG132 treatment.
It would be nice to have at least one experiment performed with an R-K mutant instead of the deletion construct.
We thus used this milder "LF" mutant instead of the "LFL" mutant in all subsequent multiple batches of neuronal culture experiments.
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Set in an alternate 1951 Britain (World War II never happened; think mutants instead of Nazis), Resistance has good looks and the polish you would expect from a game being published by Sony itself.
To further address whether the in vitro observed laddering represents poly-ubiquitination or multiple mono-ubiquitination, a number of ubiquitin mutants instead of wild-type ubiquitin were analyzed in the in vitro ubiquitination assay.
The use of hundreds of mutants instead of a few selected strains allowed a thorough comparison of these models in order to determine whether the fruit fly model can serve to predict fitness and virulence potential in the mouse.
Since these are the proteins required for the biosynthesis of class II ex-siRNAs, and given that the genes were down-regulated in the mutants instead of up-regulated, genes identified in this cluster could be secondary targets of this group of ex-siRNAs.
However, because phosphorylation of VASP inhibit its actin polymerization activity (e.g. Harbeck et al., 2000; Benz et al., 2009) the phenotypes of RNAi-rescue cells expressing phosphomimetic VASP mutants instead of wild-type VASP are expected to resemble the phenotype in VASP depleted cells.
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