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This is consistent with a folding defect and agrees with the observation that the E364K mutant induces in Drosophila the production of heat shock proteins (53).
The molecular analysis of the determinants recognized by specific antibodies and cytolytic T lymphocytes is being approached through the biochemical characterization of mutants induced in vitro and population variants that are selected by their loss of specific serological or CTL allodeterminants.
We have sequenced the genomes of yeast mutants induced in diploids by expression of the gene for PmCDA1, a hypermutagenic deaminase from sea lamprey.
We also sequenced the genomes of Canr mutants induced in an isogenic diploid strain by the powerful base analog mutagen 6-hydroxylaminopurine (HAP), one that also (like PmCDA1 in the ung1 strains) is not excised by base excision repair and does not induce recombination in yeast ([ 11, 12] and references therein).
Neither the wild type nor the constitutively active mutant induced any changes in cell cycle progression, in terms of the percentage of binucleated cells, or cell number.
We previously demonstrated that PAK1 phosphorylated EBP1 in vitro at T261 and that a phosphomimetic T261E mutant induced tamoxifen resistance in hormone sensitive MCF-7 cells (Akinmade et al, 2008).
The double I32M/E48K mutant induced significant changes in ECL1 orientation and interaction with CD81 contact residues (Fig. 1D).
Both wild-type Bid and the T59A mutant induced cell death upon expression in PC-3 cell line.
The resulting bradytrophic mutant induces during saprotrophic growth and in planta constitutively the transcription of VlcpcA encoding the cross-pathway control transcription factor.
Altogether, these results highlight the fact that the reduced QC size in the mutant induces a decrease in cell division and premature cell differentiation in the rice RAM.
It is possible that the lack of Phe66 in this mutant induces conformational disorder in Gal p, implying that Phe66 plays a role in stabilizing Gal p for nucleophilic attack.
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