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The ability to rescue the destabilized mutant indicates that circularization may be a useful tool in protein engineering programs geared towards generating minimized proteins.
The decreased membrane permeability the ΔrovA mutant indicates that the bacterial membrane was also functionally disrupted in these cells.
However, the increased expression of the same nucleotide biosynthesis genes in the dnaA46 mutant indicates that another mechanism must exist.
Downregulation of nuo genes in the phoP mutant indicates that PhoP probably controls the expression of the entire nuo operon.
The increased LD50 and lung colonization deficiency of the ppGpp null mutant indicates that ppGpp may regulate genes important in establishing a lethal infection during bubonic plague.
The filamented morphology of the nlpD mutant indicates that NlpD is involved in cell separation; however, deletion of nlpD did not affect in vitro growth rate.
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For Flavodoxin, the reduced B-factors for R125C mutant indicated that the disulfide bond further stabilizes the structure (Fig. S4D).
Up-regulated of ADL1 in rel2 mutant indicated that REL2 might have function in the pattern formation and embryo, too.
Nested exonic RT-PCR in the mutant indicated that the short forms lacked NBD1 but contained NBD2.
The expression of GA20ox2 was not decreased in slr1 mutant, indicating that the expression of GA20ox2 may not be under GA feedback regulation in slr1 mutant.
By contrast, SRL1 and ACL1 have no expression changes in rel2 mutant, indicating that the two genes may not have functional relationship with REL2 (Fig. 9).
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