The shift in affinity does not occur in a site-directed mutant in which threonine at position 286 has been replaced by a non-phosphorylatable amino acid.
Here, we characterized a NΔ15 co-expression mutant, in which the allosteric regulation was restored to wild-type levels.
We utilized the mmi1W112A mutant, in which the Mmi1 mutant protein and Erh1 are expressed at levels comparable to those in WT but fail to form the EMC.
One can imagine an early bombardier like M. contractus which produced froth on the outside of its body throwing up a mutant in which the froth-making machinery became enclosed in a body cavity.
Using the Arabidopsis kpl mutant, in which single-fertilization events are frequent, we showed that endosperm development is not required for pattern formation during early embryogenesis.
Analysis of a mutant in which the linker "undocks" from the ring indicates that linker remodeling requires energy that is supplied by interactions with the AAA+ modules.
A D148C mutant, in which Asp148 was replaced with Cys, was designed to enhance stability and folding in low salt solution by S S bond.
We produced a synthetic PA mutant in which an α helix was crosslinked into the α clamp to block its function.
IL13.K105R mutant, in which lysine was substituted by arginine, neutralized the killing of IL13Rα2-positive cells by IL13-based cytotoxin more efficiently than wild-type IL13.
To test this hypothesis, CS-HA1ΔGly, a mutant in which the unique N-glycosylation site Asn316 was changed to Ile, was engineered by site-directed mutagenesis.
In this work, we analyze the mechanical stability of a BR triple mutant in which three key extracellular residues, Glu9, Glu194, and Glu204, were mutated simultaneously to Gln.
