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To this end, we generated a respiration-deficient (RD) mutant in order to enforce engineered S. cerevisiae to utilize xylose only through fermentative metabolic routes.
This work analyses a recently published microarray data of a Dam mutant in order to assess the effect this has on transcription regulation.
Therefore, in this study we also used the W241A mutant, which has no detectable effect on GTP binding (Fig. 2), in addition to the C277S mutant, in order to determine whether these two mutations have differential effects on the role of TG2 in cell death.
As RovS was shown to directly bind to the promoter of fbsA and hence to negatively regulate its transcription [24], we quantified rovS transcript levels in a ΔrgfAC mutant in order to determine whether RgfA/RgfC regulated fbsA through rovS gene.
We also generated a sakA deletion mutant in order to assess SakA/AtfA interactions.
However, Rad2C65Δp was expressed in rad2Δ mutant in order to avoid any possible phenotypic interference from the genomic Rad2p in WT background.
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We utilized the close homology between IA-2 and IA-2β PTP domains to design chimeras and mutants in order to identify humoral IA-2-specific epitopes.
For this reason, we performed oscillatory shear experiments with six different desmin "tail" mutants in order to characterize the mesh size of filament networks and their strain stiffening properties.
Gene guns and mutagenesis techniques insert or disrupt genes randomly in the plant genome, such that plant researchers must make and screen thousands of mutants in order to find one with the right change in the right place.
While Charles begins to recruit other mutants in order to confront this menace, one Erik Lehnsherr Michael Fassbenderr), a powerful mutant with a personal vendetta against Shaw, will enter the scene and change the face of mutant/human relations forever.
As stated in the Materials and Methods, the C177A mutation was also introduced in all of the above mutants in order to reduce aggregation of the recombinant protein.
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