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However, only a mutant in one LPS biosynthetic gene (rfaC) was indentified among the Keio collection.
These paralogous genes probably make different contributions to pH homeostasis as a CIAT 899 mutant in one channel (sycA) was apparently not impaired in growth at pH 4.5, but rather showed symbiotic defects [ 25].
To assess the potential in vivo benefits of slower metabolism in cells with a high oxidative load, we used a mutant in one of the main H2O2 detoxification enzymes, TSA1, known to be prone to high CIN (Ragu et al. 2007).
Recently, a pea deletion mutant in one HD-Zip protein, in which tendrils were converted into leaflets (they were no longer inhibited from completing laminar development), was shown to exhibit the same phenotype as a mutant in which the 12 amino acids of its CTR were not translated [ 38].
Also despite their repression by cold treatment, a loss-of-function mutant in one of these enzymes (CHY1) was recently shown to be more sensitive to freezing following cold acclimation [64].
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In the first round clone-pools were examined containing all mutants in one microtiter plate.
The mutants in one cluster all affect electrostatic interactions between stacked subunits in the long pitch helix of the actin filament.
Suppose we write n G and n P for the numbers of good and poor quality mutants in one generation, and n G ′ and n P ′ for their numbers in the next.
Mutants in one myoinositol-1-phosphate synthase (AtIPS1) exhibit accelerated cell death [ 13]; the encoded protein has a nuclear localization sequence, suggesting that nuclear pools of myoinositol may be critical [ 13].
Genome-wide, at least 5500 genomic intervals to which 24 nt siRNAs map have overlapping 26-94 nt P4R2 RNAs that are detectable in Col-0, and typically increase in abundance in dcl2/3/4 mutants, but are absent, or substantially reduced, in pol IV or rdr2 null mutants in one or both biological replicates (Supplementary file 1).
Consistent with this view is the fact that virus mutants severely impaired for the initiation of lytic cycle, such as mutants in one or more IE genes or VP16 (Chiocca et al., 1990; Dobson et al., 1990; Katz et al., 1990; Steiner et al., 1990; Ecob-Prince et al., 1993; Sedarati et al., 1993; Marshall et al., 2000) are able to establish latent infection in vivo.
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