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Introduce one S mutant in each island of the first group and one A mutant in each islands of the second group.
Future analysis of mice with conditional expression of the mutant in each type of neuron could address the impact on each directly.
The allelic burden of the ITD mutant in each patient ranged from 2.3 to 75.2% with a median of 32.9% (Table 2).
The data for the alternate CLS screen with cells re-grown before Bar-seq analysis are provided in Table S5 (sequence counts per mutant in each sample) and in Table S6 (long-lived mutants unique for re-growth screen).
We performed two independent biological repeats of the mutant pool grown exponentially in EMM for 9 hr, with samples for Bar-seq analysis harvested at 5 timepoints between 120 and 550 min. The sequence counts for each mutant in each sample are provided in Table S7.
In Table 2, panels b and c, the overall HPRT M f from these individual studies and subpopulations (panel b), or the M f generated from our pooled analysis (panel c), are used to calculate the frequency of each type of mutant in each population, as was done in Manchester et al [ 21] and Finette et al [ 23].
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Single mutants in each of the genes exhibit reduced numbers of the enlarged and diffuse postsynaptic receptor clusters.
Next, we performed lethal complementation tests between xen72−3 and mutants in each of the eight genes that are deleted in Df 2R 03072.
We treated mutants in each of the three major apoptosis pathways with misc-1 RNAi and assessed apoptosis levels by SYTO12 staining (Fig. 4D).
We also ranked the transcripts that did not change in abundance amongst the mutants in each of the 23 tissues to obtain the expected distribution of gene expression ranks within each tissue.
Second, WT α-syn, A53T and E46K (but not A30P) were extremely toxic to the three elongase mutants, in each case yielding >10-fold decrease in survival (t50%: 7-8 d →0.5 d) compared to the same cells with empty vector.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com