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This pattern of lengths of cell cycles was not observed in embryos mutant in cell cycle factors.
Studies later demonstrated that Hfq was involved in Salmonella pathogenesis through its effect on expression and secretion of virulence factors (in a σS-independent manner) resulting in an attenuation of the Salmonella hfq deletion mutant in cell culture system as well as in murine model [28].
Secondly, aggregation associated with the expression of the P182L-HSPB1 mutant in cell models was significantly reduced by the overexpression of wild-type HSPB8 (Carra et al., 2010).
Checking the level of VEGFR2 phosphorylation in response to VEGF and an assessment of VEGFR2 trafficking with vs. without Nrp1 vs. with the Nrp1 VEGF binding mutant (in cell culture) would be very helpful.
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Our finding that CBP stabilizes WRN through acetylation has been further examined by expressing WRN acetylation mutant in cells.
The analysis by Native-PAGE also showed that most forms of the C9/20/53/63/91A mutant in cells are monomer, but not dimer.
We expressed the dominant-negative dynamin-K44A mutant in cells treated with EHD2 siRNA.
Conversely, we asked whether expression of a Beclin 1 S90E phosphomimetic mutant in cells lacking detectable endogenous Beclin 1 could bypass the inhibitory effects of dominant negative MK2.
When we expressed the mutant in cells, we found that it exhibited a diffuse cytoplasmic localization and did not concentrate on the cortex.
However, the activities of the M-1, M-3, M-4, M-5, and M-6 mutants in cell lysate were lower than that of wild-type CueO (Fig. 2).
The pcDNA plasmid containing the Z (E342K) α1-antitrypsin allele was used as the basis for the mutants in cell culture experiments [ 29].
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